Process development of succinic acid production from lignocellulosic materials by Actinobacillus sp.

Abstract

Total 30 samples were collected from soil and bovine dung in Surin (SR1-2), Suphanburi (SP1-10), Nakornsawan( NS1-12) and Nakhonprathom (NP1-6) province, Thailand. Two hundreds thirty bacterial isolates with succinic acid ability were obtained. Two hundreds and sixteen isolates were gram-positive and fourteen isolates were gram negative bacteria. Two of these negative isolates, isolate SP8/A4 and NS2/A2, have a potential for succinic acid production with 42.08 and 43.26 g/l, respectively. Actinobacillus succinogenes DSMZ 22257 was used as a representative for succinic acid production from lignocellulosic material. The use of 60 g/l (equivalent reducing sugar) sorghum straw hydrolyzate as a carbon source, longer lag phase was found and the highest amount cell growth of 2.665 g/l and succinic acid of 13.820 g/l were obtained. When using glucose 60g/l as a carbon source, a maximum cell growth of 3.185 g/l and succinic acid 44.799 g/l were obtained.